#Polytool nitrogen full#

Samples are introduced via a Waters Acquity H-class UPLC system with flow injection or column separation, or by infusion, and ionized with ESI or APCI sources. Bruker maXis UPLC Q-TOF MS, the‘maXis’: MaXis is primarily used for accurate mass analysis, intact protein analysis, structure determination and untargeted characterization.Software: Waters Mass Lynx, Target Lynx training for open-source Skyline data processing is also available. The triple quadrupole mass analyzer performs mass selective fragmentation (MS/MS) for quantitative experiments with unit resolution and nominal mass accuracy. Samples may also be introduced as a dried spot for ASAP ionization. Ionization may be done with ESI or APCI sources. Samples are introduced via a Waters Acquity H-class UPLC system with flow injection or column separation, or by infusion. Waters Xevo UPLC TQD MS, the ‘Xevo’: The Xevo is primarily used for targeted quantitative assays.The TOF mass analyzer provides accurate mass data with resolution 18,000 FWHM for m/z 1,522, and mass accuracy of +/-5 ppm. Samples may alternatively be introduced as a dried spot for DART ionization. Ionization can be performed with ESI, APCI or MMI (multimode ionization-combined ESI and APCI) sources. Samples are introduced via an Agilent 1290 UPLC system with flow injection or column separation, or by infusion. Agilent 6230 UPLC TOF MS, the ‘B-TOF’: The B-TOF is primarily used for accurate mass analysis.

#Polytool nitrogen full#

A full list of MS-based assays developed in the ARC can be found by clicking here.Untargeted Analysis: data-dependent, or data-independent acquisition of spectra processed with chemometric tools to understand changes in small molecule profiles in complex mixture under experimental or observational conditions.Targeted Quantitative Analysis: relative or absolute quantitation of specific analytes.Biotyper Analysis: rapid and high throughput bacterial identification method using MALDI-TOF that compares the MS “fingerprint” of a bacterial colony to databases that contain the spectra of thousands of known bacterial species.Polymer Characterization: molecular weight characterization (from 0.5 kDa up to 500kDa) and end group analysis using MALDI-TOF for polymers that are generally difficult to ionize through other means.Intact Protein Analysis: molecular weight confirmation and detection of protein modifications.Molecular Identification and Structure Determination: accurate mass analysis for the generation and confirmation of molecular formula and MS fragmentation patterns.